Analysis Note

ControlHeLa cell lysate

Application

Research Sub CategoryRNA Metabolism & Binding Proteins

Research CategoryEpigenetics & Nuclear Function

Anti-Gemin3 Antibody, clone 12H12 is a Mouse Monoclonal Antibody for detection of Gemin3 also known as Component of gems 3, DEAD-box protein DP103 & has been validated in WB.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Gemin3 is a member of the DEAD box protein family. The members of this family are characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD). Some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. Gemin 3 is a component of the survival of motor neurons (SMN) complex and codes for a DEAD box protein with an ATPase activity The SMN complex is essential for the biogenesis of spliceosomal small nuclear ribonucleoproteins and likely functions in the assembly, metabolism, and transport of a diverse number of other ribonucleoproteins. Specifically, the SMN complex assembles 7 Sm proteins (Gemin2-8) into a core structure around a highly conserved sequence of ribonucleic acid (RNA) found in small nuclear RNAs. The complex recognizes specific sequences and structural features of small nuclear RNAs and Sm proteins and assembles small nuclear ribonucleoproteins in a stepwise fashion.

Immunogen

Epitope: Unknown

Recombinant protein corresponding to human Gemin-3.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified mouse monoclonal IgGκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Protein G Purified

Format: Purified

Quality

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.5 µg/ml of this antibody detected Gemin-3 on 10 µg of HeLa cell lysate.

Specificity

This antibody recognizes Gemin-3.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

Detects a band of approximately 105 kDa (Calculated molecular weight: 92 kDa)